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INSULIN :FROM EGYPTIAN TILAPIA BROCKMANN BODIES
Volume: 22
Number: 22
Year: 2014
Pages: 27-34
FATTAH, Mohy Ei Deen Abdel; MOHAMED, NAGWA Ibrahim ; ASBREY, Heba El
Abstract:
<p align="justify"> Certain teleost fish have large anatomically discrete islet orga11s called Brockmann bodies (BBs) that are much more easily harvested than pancreatic islets. Brockmann bodies (islet organs) of both types were excised from freshly killed tilapia fish at the Fisheries Center, Faculty of Agriculture of lsmailia, and immediately frozen. Approximately 5.1 g of islet tissue was obtained from 81 fish of approximate weight that ranged from 150-350. These Brockmann bodies (BBs) were subjected to acid/alcohol extraction and gel filtration. Ultraviolet and infrared spectral measurements were performed. The fraction with the highest absorption was studied further and compared to standard insulin. They both showed the presence of alcohol/phenol OH groups and the amide C=O stretch. </p>
DOI: 10.48141/SBJCHEM.v22.n22.2014.30_revista2014.pdf
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