Abstract:
In order to investigate the antioxidant activity of Salvia nemorosa L. collected from Ahar and Urmia regionsin Iran at different growth stages, aerial parts of sage after collecting were dried, and for measurement, the abilityof scavenge DPPH (2,2-diphenyl-1-picryl-hydrazyl-hydrate) radical in different concentrations (0.025, 0.05, 0.07,0.1, 0.2, 0.04 and 0.6) of methanolic extracts were prepared. The result showed that the ability to scavenge DPPHradical and amount of inhibition percent of vegetative stage leaves, flowering stage leaves, and flowers increasedwith increasing concentrations of methanolic extracts from 0.25 to 0.6 mg/mL. In the region of Urmia, the highestamount of DPPH inhibition there was in vegetative stage leaves, and the lowest amount of DPPH inhibition wasseen in flowers. In Ahar regions, unlike the Urmia region, the highest amount of DPPH inhibition there was inflowers, but the lowest of DPPH inhibition was seen in flowering stage leaves. Also, the content of inhibition ofDPPH in Ahar and Urmia regions similarly increased between two phenological stages (vegetative stage leaves,flowering stage leaves, and flowers) in 0.4 mg/mL and 0.6 mg/mL concentrations.