IMMOBILIZATION OF ALCOHOL OXIDASE IN SiO<sub>2</sub> MATRIX PREPARED BY SOL-GEL METHOD
Abstract:
<p align="justify"> The possibility of obtaining a biochemical sensor for colorimetric determination was studied for this purpose, we have used the redox indicator 2.6-dichlorophenolindophenol (DCIP) and the binary enzymatic system composed by alcohol oxidase (AO) and peroxidase (PER). The binary enzymatic system immobilized in an inert SiO<sub>2</sub> matrix was obtained by modfied sol-gel process. The chromogen and binary enzymatic system immobilization into a SiO<sub>2</sub> matrix was confirmed by I.R. spectroscopy. By immobilization of the enzymatic system, both the stability and enzymatic activity increase. </p>
<p align="justify"> The possibility of obtaining a biochemical sensor for colorimetric determination was studied for this purpose, we have used the redox indicator 2.6-dichlorophenolindophenol (DCIP) and the binary enzymatic system composed by alcohol oxidase (AO) and peroxidase (PER). The binary enzymatic system immobilized in an inert SiO<sub>2</sub> matrix was obtained by modfied sol-gel process. The chromogen and binary enzymatic system immobilization into a SiO<sub>2</sub> matrix was confirmed by I.R. spectroscopy. By immobilization of the enzymatic system, both the stability and enzymatic activity increase. </p>
DOI: 10.48141/SBJCHEM.v6.n6.1998.86_1998.pdf
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