EQUILIBRIUM AND HYDROLYSIS STUDIES OF PHOSPHATE ESTERS MODEL MOLECULES AND DNA CATALYZED BY OBISDIEN-Zn(II) COMPLEXES.
Abstract:
<p align="justify"> Dinuclear Zn(II)-OBISDIEN complexes catalyze hydrolysis of DNA and a phosphate ester model molecule: bis(2,4-dinitrophenyl)phosphate (BDNPP). The increase in the rate of hydrolysis of BDNPP at pH values above 8.0 is attributed to the presence of a ternary hydroxide species, in which intramolecular catalysis is favored by the proximity of the hydroxide group coordinated to the bimetallic center of the receptor complexes, in agreement with equilibrium results of OBISDIEN-Zn(II)-ATP (1:2:1 molar ratio) system. In the treatment of pBR322 circular DNA with OBISDIEN-Zn(II) complexes, the results suggest that these complexes cleave DNA in a random and nonspecific manner since no distinguishable low molecular weight bands were observed after treatment </p>
<p align="justify"> Dinuclear Zn(II)-OBISDIEN complexes catalyze hydrolysis of DNA and a phosphate ester model molecule: bis(2,4-dinitrophenyl)phosphate (BDNPP). The increase in the rate of hydrolysis of BDNPP at pH values above 8.0 is attributed to the presence of a ternary hydroxide species, in which intramolecular catalysis is favored by the proximity of the hydroxide group coordinated to the bimetallic center of the receptor complexes, in agreement with equilibrium results of OBISDIEN-Zn(II)-ATP (1:2:1 molar ratio) system. In the treatment of pBR322 circular DNA with OBISDIEN-Zn(II) complexes, the results suggest that these complexes cleave DNA in a random and nonspecific manner since no distinguishable low molecular weight bands were observed after treatment </p>
DOI: 10.48141/SBJCHEM.v7.n8.1999.14_1999.pdf
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