COMPARATIVE STUDY OF DPD REAGENTS FOR CHLORINE MEASUREMENT IN DRINKING WATER AND DEVELOPMENT OF A JAVASCRIPT INTERPOLATION TOOL
Background: Determining chlorine in water ensures safety. Among other methods, the DPD colorimetric method is used. The DPD Method relies on colorimetric reactions to measure free and total chlorine concentration in water samples with pink compound formation. Aims: To perform a comparative chlorine analysis using DPD, assessing reagents from 3 makers and 2 Hach instruments to identify disparities and propose adjustments for more accurate measurements. Methods: Hach High-Range and Low-Range Free chlorine determination procedures were followed. DR300 and POCKET Colorimeter II spectrophotometers were used. Tests were conducted for each DPD manufacturer in low/high ranges and in two HACH devices to determine the chlorine concentrations. Hach was used as the reference; LaMotte and PoliControl compared against it. Statistical analyses were compiled using MS Excel. Results: The tests findings were gathered in Tables 1-5. JavaScript and HTML scripts were created to convert LaMotte and PoliControl outcomes into values equivalent to those of HACH through linear interpolation. Discussion: Various DPD reagents and equipment provided slightly different readings, prompting empirical evaluation of these differences. Adjusting the results to Hach's results was selected as both the reagent and spectrophotometer were from the same brand. Differences in spectrophotometer readings were more pronounced in high-range tests nearing the upper limit of the test. Conclusions: Equipment variations caused minor result differences; DPD reagents are not interchangeable without correlation. The Open-source code developed aided in reducing reading disparities.
Read ArticleE-SELECTIN AS A BIOMARKER IN FEMALE PATIENTS WITH Β-THALASSEMIA IN AL- NAJAF PROVENCE, IRAQ
E-selectin, as identified (CD62E), is expressed on endothelial cells after stimulation with inflammation cytokines. β-Thalassemia diseases (βT) and early diagnosis are of utmost significance in the entire world population. This study was performed in the Thalassemia Center of the Al-Zahraa Educational Hospital in Al-Najaf Province, Iraq, on sixty-nine with β-thalassemia (54 βT major and 15 βT Intermedia) aged 8-40 years who transfused blood. Compared to 20 healthy volunteers as a control group. In both βT patients and healthy groups were assessed serum E-selectin levels. It was investigated the relationship with RBC, Hb, PCV, WBC, PLT, BMI, splenic status, iron, and ferritin levels. The results revealed a significant (P<0.05) decreased values of HB, RBC, P.C.V, and BMI. In contrast, values of WBC, PLT, Iron, and Ferritin were significantly increased in βT patients as compared to the healthy control groups. A significant (P<0.05) increase in serum E- Selectin level in βT patients (20.55±0.47) ng/ml to compare with the healthy group (9.16±0.50) ng/ml. Furthermore, it was a significant decrease in groups of βT major (19.87±0.42) ng/ml more than in βT intermedia (23±1.42) ng/ml. E-Selectin revealed a significant increase (P<0.05) in progress age and associated with splenectomies and underweight groups compared to splenectomies and the normal weight groups, respectively. Also, E-Selectin levels significantly positively correlated with WBC, PLT value, iron, and Ferritin levels. However, it was no significant with RBC, PCV, Hb. As a conclusion from this study, E- Selectin is an important biomarker in β-thalassemia patients can be identified as the complications associated with iron overload, inflammatory process, and endothelial dysfunction in βT disease.
Read ArticleGEOSPATIAL IDENTIFICATION OF BUILT-UP STRUCTURES ALONG THE DISCERNED KAZAURE–KARAUKARAU–KUSHAKA–ILESHA SCHIST BELT
Background: The desire to create a database of research documents providing information about the tracts of gold deposits across the local geological province provides the impetus for a study of the kind being considered here. Geospatial identification of built-up structures within Phase I Development along the Kazaure-Karaukarau-Kushaka-Ilesha Schist Belt, trending through Minna town and its outlying districts, constitutes the veritable reference material desired in this regard. Methods: This study began by segmenting the area of study for ground-based and remotely sensed attribute mapping, using the key reference map from a previous study as the area-of-study guide. A handheld Garmin GPSmap78® global positioning system unit and a standard smartphone with a built-in camera were the key equipment used for the fieldwork. Polygonal-format georeferenced coordinate information was collected at conveniently detached buildings, beginning with the cluster of residential homes at the Staff Quarters, for the ground-based survey. Result: Nine of the ten built-up structure clusters on the path of the Belt in Phase I Development were mapped for this study, as well as six neighborhoods in the Minna built-up area beyond Phase I. The nine clusters occupy almost 40% of the circa 2 km2 areal extent of the Phase I Development. The belt's trend and structures were north-northeast. Discussion: The nine cluster structures in Phase I and the six neighborhoods of Minna identified in this study have been determined to align with the path of the belt. Conclusion: Having now determined that the trace of the belt exits the Campus at the northern sector of the Gidan Kwano village and trends in a long arc beyond the town, this study becomes the desired reference material to be archived and consulted for information relating to gold exploitation in the Minna Area geological province.
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