E-SELECTIN AS A BIOMARKER IN FEMALE PATIENTS WITH Β-THALASSEMIA IN AL- NAJAF PROVENCE, IRAQ
E-selectin, as identified (CD62E), is expressed on endothelial cells after stimulation with inflammation cytokines. β-Thalassemia diseases (βT) and early diagnosis are of utmost significance in the entire world population. This study was performed in the Thalassemia Center of the Al-Zahraa Educational Hospital in Al-Najaf Province, Iraq, on sixty-nine with β-thalassemia (54 βT major and 15 βT Intermedia) aged 8-40 years who transfused blood. Compared to 20 healthy volunteers as a control group. In both βT patients and healthy groups were assessed serum E-selectin levels. It was investigated the relationship with RBC, Hb, PCV, WBC, PLT, BMI, splenic status, iron, and ferritin levels. The results revealed a significant (P<0.05) decreased values of HB, RBC, P.C.V, and BMI. In contrast, values of WBC, PLT, Iron, and Ferritin were significantly increased in βT patients as compared to the healthy control groups. A significant (P<0.05) increase in serum E- Selectin level in βT patients (20.55±0.47) ng/ml to compare with the healthy group (9.16±0.50) ng/ml. Furthermore, it was a significant decrease in groups of βT major (19.87±0.42) ng/ml more than in βT intermedia (23±1.42) ng/ml. E-Selectin revealed a significant increase (P<0.05) in progress age and associated with splenectomies and underweight groups compared to splenectomies and the normal weight groups, respectively. Also, E-Selectin levels significantly positively correlated with WBC, PLT value, iron, and Ferritin levels. However, it was no significant with RBC, PCV, Hb. As a conclusion from this study, E- Selectin is an important biomarker in β-thalassemia patients can be identified as the complications associated with iron overload, inflammatory process, and endothelial dysfunction in βT disease.
Read ArticleUTILIZATION OF PROTEOMICS APPROACH TO UNDERSTAND GENES ASSOCIATED WITH THE OCCURRENCE OF BIOTIC STRESS IN PLANTS
Of the most fundamental fields of modern biology is transcriptomics, with a focal point on the expression pattern of plants under various conditions by assessing ribonucleic acid. So far, this approach has been a game-changer in revealing the gene structure, function, and most importantly, their cellular and biological role. Considering the criticality of pathogens for crop plants, understanding plant defense mechanisms against them is in high demand. This study aimed to review the principles of these approaches and their recent application in the plant. An Important method to address this gap is transcriptomics, which can effectively provide insight into plants against pathogens. This field has covered different aspects of plant biology besides the plant-pathogen relationship. Identifying pathogens in infected plants and the series of reactions they provoke at the gene level is crucial to finding the responsible gene (s). Finding the gene associated with resistance or vulnerability to a specific pathogen paves the way to differentiate the potential genotypes. Thus, the breeding attempts would be more successful. The advancement in biotechnology has revolutionized this field with some of the methods that have been commonly applied in studies on the plant-pathogen relationship, for instance, Northern blotting, microarray, real-time polymerase chain reaction.
Read ArticleEXTRACTION AND CHARACTERIZATION OF CURCUMIN FROM TURMERIC RHIZOMES GROWN IN MÉRIDA, VENEZUELA
The extraction of naturally occurring compounds is one of the fastest-growing industries because of its benefits against its synthetic analogs. Environmental protection must require the use of natural products instead of chemicals to minimize pollution. Thus, this investigation studies the use of some natural product, as curcumin, as naturally occurring acid‐base indicators. Curcumin can be used as acid-base indicators since it was found that it possesses pH-dependent solubility. Curcumin, the major active component of turmeric, Curcuma longa (Zingiberaceae), is used as a spice in curry and as a coloring agent in yellow mustards, cosmetics, pharmaceuticals, and hair dyes. In this research, the main compound colored rhizome of turmeric (Curcuma longa) cultivated in Mérida, Venezuela, is extracted: Curcumin (C21H20O6) (1E,6E)-1,7-bis(4-hydroxy-3-methoxyphenyl)-1,6-heptadiene-3,5-dione, in a yield of 3.42% after 8 hours of extraction using soxhlet extractor system with organic solvents (hexane and ethanol). The thin-layer chromatography and column performed separation and purification using a mobile phase, a mixture of chloroform-hexane 3:2. The dye was characterized by spectroscopic analysis of visible ultraviolet (UV-Vis) and infrared (IR), in addition to his studio in steering sensitivity as an acid-base indicator. This dye is useful as an acid-base indicator in strong acid-strong base volumes and did not require large amounts of it as it has high sensitivity. The results indicate that curcumin as an acid-base indicator allows the development of new standards in different chemistry fields that require this type of analysis.
Read Article