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DETECTION OF EPSTEIN-BARR VIRUS (EBV) IN WOMEN WITH BREAST CANCER IN IRAQ USING IN-SITU HYBRIDIZATION AND IMMUNOHISTOCHEMICAL TECHNIQUES

Background: The Epstein-Barr virus (EBV) has recently been identified in human breast cancer globally, potentially contributing to the initiation and progression of this malignancy, as well as gastric cancer, nasopharyngeal carcinoma, and bladder cancer. It has been newly associated with breast cancer. Globally, breast cancer affects more women than any other type of cancer. In Iraq, the prevalence of breast cancer is comparable. Aims: The study examined Iraqi women diagnosed with invasive ductal carcinoma (IDC) and invasive lobular carcinoma (ILC) to detect Epstein-Barr Virus Nuclear Antigen-1 (EBNA-1) and encoded RNA (EBER). Methods: A total of 50 formalin-fixed paraffin-embedded tissues from invasive ductal carcinoma (IDC) (92%) and invasive lobular carcinoma (ILC) (8%) biopsy samples constituted the case group, while 30 formalin-fixed paraffin-embedded tissues from non-cancerous breast tissue served as the control group. The presence of Epstein-Barr virus protein (EBER) in breast tissue was assessed using immunohistochemistry (IHC) and chromogenic in situ hybridization (CISH) methods. Results: EBER RNA signals were found in 31 (62%). EBER RNA signals were seen in 3 (10%) control group participants. Significant differences (P<0.04) were seen in EBV EBER RNA positive signals among study groups. Immunohistochemistry showed nuclear brown staining in 34 (68%) breast cancer patients. Control group: 3 (10%). Discussion: The research identified a statistically significant correlation between EBV positivity and breast cancer among Iraqi women, especially concerning invasive ductal carcinoma. The results corroborate previous reports of elevated EBV levels in malignant breast tissues relative to controls. Although detection approaches such as CISH and IHC provide complementary insights, additional studies are needed. Conclusions: The study concludes that EBNA-1 and EBV EBER RNA were overexpressed in our population group.
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INTERVIEW WITH DEPUTY VICE CHANCELLOR DR. O. A. OMOTESHO, UNIVERSITY OF ILORIN, NIGERIA (ENGLISH VERSION)

Background: The University of Ilorin, founded in 1975 in Nigeria, has evolved from 3 to 16 faculties, becoming the country's most sought-after institution for the past two decades. Aims: To document the institutional evolution, identify the most demanded programs, assess scientific output, examine internationalization strategies, and understand strategic development objectives. Methods: Structured interview with Vice-Chancellor Dr. Olubumi Abayomi Omotesho, following a standardized protocol covering historical, academic, scientific, and strategic aspects of the institution, under Creative Commons license format. Results: The university expanded to 16 faculties in 49 years. The most demanded programs are Medicine and Nursing, followed by Pharmacy, Law, Engineering, and Accounting. Areas with the highest scientific output: Medicine, Biological/Agricultural Sciences, and Engineering. It offers 340 postgraduate programs with approximately 7,523 students. There is a dedicated infrastructure for internationalization, with plans for international accommodations. Discussion: The predominance of healthcare courses reflects global employability trends. Research aligned with Sustainable Development Goals demonstrates a contemporary vision. The institutional goal (number one in Nigeria, top 10 in Africa, top 500 globally) shows a measurable strategic approach. Commitment to internationalization aligns with global education trends. Conclusions: The institution exemplifies an evolving African university focused on academic excellence, scientific relevance, and internationalization. The prioritization of student-centered development, clear positioning goals, and international collaboration initiatives establish solid foundations for its contribution to regional and global knowledge.
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INQUIRY FOR SUITABLE LOCATIONS FOR A DRILLING REGIME AT AN UPSLOPE ROCKY KNOLL OF LAWU ESTATE, WESTERN BYPASS, MINNA, NIGERIA

Background: A client requested that the study group help determine locations that would be suitable for a drilling regime at his lot, located at an upslope rocky knoll of Lawu Estate, Minna, Nigeria. Aim: The aim of this study is to carry out a purpose-specific survey to pinpoint the best locations in a built-up property at the upmarket Lawu Estate that would be suitable for a drilling regime targeted for household consumption. Methods: The study area was reconnoitered by the survey crew in order to georeference the locations that would be occupied for the vertical electrical sounding survey in the 30 m x 20 m lot. Owing to the extensive build-up at this lot, only a four-point traverse along the 30-metric dimension traverse of the frontage of the building was demarcated in the northeasterly direction, thereby limiting the desire of the survey crew to define an appropriate survey grid. The data-acquisition pattern at the 4 x 1 survey stations of the frontage-traverse of the lot followed the “traditional” sequence of Schlumberger array layout measurements, whence the survey crew progressed with current-electrode spacing either end of a survey point located at this frontage-traverse targeting a maximum survey depth of 100 m. Result: The acquired vertical electrical-sounding data set for this study was recorded on purpose-specific data sheets. Discussion: Based on empirical rules-of-thumb procedures for interpreting vertical electrical sounding data at the Nigerian Basement Complex geological province, “assured” groundwater location and “strongly aquiferous” location, deductive inferences were drawn with regards to only vertical electrical sounding Station 4. Conclusion: Thus, it is recommended that VES Station 4 be exploited in the planned drilling program of the client, especially since this survey point checks off 100 percent of the constraints imposed by the rules-of-thumb interpretation procedures.
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General information

SOUTHERN JOURNAL OF SCIENCES

    General information about this journal
  • Title: SOUTHERN JOURNAL OF SCIENCES
  • Short Title: South. J. Sci.
  • ISSN: 2764-5959 (Online); ISSN: 2764-5967 (Print)
  • Universal Decimal Classification (UDC): 001
  • Review Process: Double-Blind Peer-Review
  • Accessibility: Platinum Open Access, NO-APCs.
  • Digital preservation: Portico
  • Frequency of Publication: biannual [2 issues per year]. Journal publication schedule
  • DOI: 10.48141/2764-5959
  • Website: https://www.sjofsciences.com/
  • Country: BRAZIL
  • Publisher: Araucária - Scientific Association.
  • Language of Publication: ENGLISH / PORTUGUESE*
  • *Year that the Journal started accepting manuscripts in Portuguese: 2020
  • First issue year: 1993
  • Free full text: Yes
  • Indexed in: Index Copernicus; Latindex, and I2OR.
  • Formerly known as the Southern Brazilian Journal of Chemistry (1993 to 2021).
  • Former ISSN: 2674-6891 (Online); Former ISSN: 0104-5431 (Print).
  • Website last update: 06/07/2025.

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COMPARATIVE STUDY OF ALCOHOLIC EXTRACTION OF COMPOUNDS FROM ARAUCARIA ANGUSTIFOLIA: MICROWAVE-ASSISTED EXTRACTION VS. MACERATION EXTRACTION

Background: The Araucaria angustifolia is a prominent tree species indigenous to the southern regions of Brazil. The tree can be a source of different compounds of interest if properly used. Aims: This research aims to compare the alcoholic extraction of compounds from Araucaria angustifolia using two different methods, microwave-assisted extraction and maceration extraction. Methods: Alcahoolic extractions of compounds from the sawdust of Araucaria angustifolia were performed using microwave-assisted extraction and maceration extraction. The color was observed by eye inspection. The taste was observed by putting the samples into the mouth and tasting it. The olfactive test was conducted at hot and cold temperatures. Results: it was obtained red color solution, both from the bark and from the branch. The intensity of the color changed with the extraction method and time of maceration. The solutions tasted like "green wood". Discussion: MAE and Maceration extraction can provide extracts from the bark and branch of the araucaria tree. There is the possibility that the “green wood” taste of the MAE extract can be changed with the thermal treatment of the wood. Conclusions: microwave-assisted extraction can perform the faster extraction of compounds from Araucaria angustifolia than maceration extraction.
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EXPERIMENTAL STUDY OF THE INFLUENCE OF TEMPERATURE ON PASTEURIZATION OF PÊRA RIO IN NATURA ORANGE JUICE

Background: Heat treatment is one of the most used methods to preserve food, such as orange juices, which are an excellent source of ascorbic acid. To avoid vitamin C degradation and reduce loss, fast heating is recommended. However, little is known about heat transfer during juice pasteurization. Aim: Therefore, this work aimed to determine the vitamin C content and the convective heat transfer coefficient in the pasteurization of orange juice. Methods: To perform the experiment, in the juice container, two regions were analyzed: the central region and near the wall. For the time-temperature control, thermometers were installed in the two regions mentioned. Every 120 seconds, the temperature was measured. The vitamin C content in the juice was evaluated before and after pasteurization using the iodometric method. The convective coefficient was evaluated using the method of dimensionless numbers and the experimental method. Results and Discussion: In pasteurization, the solution was heated to 80 °C, where heating lasted 3000 seconds and cooling for 2520 seconds. The graph showing the relationship of the convective heat transfer coefficient and temperature follows the same trend of the literature. The convective coefficient is higher in the region near the wall. As time passes and temperature decreases, the central region tends to equilibrium, and the coefficient becomes more constant. The vitamin C content remained constant before and after pasteurization. The values of the dimensionless numbers used in the calculations are in the same order of magnitude as the literature. Conclusions: The pasteurization did not cause ascorbic acid degradation since the heating step was fast in the heat treatment. The graphic showed that there is a dependence of the dimensionless of temperature with the dimensionless Biot and Fourier. It was noted that studying the thermal behavior in the cooling of orange juice is extremely important to ensure its quality.
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STUDY ON THE DPPH FREE RADICAL-SCAVENGING ACTIVITY OF SALVIA NEMOROSA L. AT TWO GROWTH STAGES

This study was designed to examine the DPPH free radical-scavenging activity, in different concentrations (0.025, 0.05, 0.07, 0.1, 0.2, 0.04 and 0.6) of methanolic extracts of Salvia nemorosa L. collected from the northwest of Iran (Zonouz and Ardabil regions) at two-stage of growth (vegetative stage leaves, flowering stage leaves, and flowers). The result showed that the mean of inhibition percentage in the Zonouz region increased in various concentrations and between flowers, vegetative stage leaves, and flowering stage leaves, compared with the plants of the Ardabil region. In each of the regions of Zonouz and Ardabil, the highest amount of DPPH inhibition was observed in the vegetative stage leaves in comparison with flowering stage leaves and flowers. In addition, in the effect of DPPH radical trapping in different concentrations of methanolic extracts of Salvia nemorosa L. was observed that from each of the collected region, methanolic extracts from sage plants were dose-dependent and acted very effective and useful and the best antioxidant activity was in the high concentration of extracts, So in Zonouz and Ardabil regions, the content of inhibition of DPPH increased significantly, by increasing the concentration of 0.025 mg/ml to 0.6 mg/ml and in Zonouz region the content of inhibition of DPPH similarly increased in 0.2, 0.4 and 0.6 mg/ml concentrations. In the Ardabil region, the most content of inhibition of DPPH was seen in 0.4 mg/ml and 0.6 mg/ml concentrations, but in this region, the content of inhibition of DPPH in 0.2 mg/ml concentration there was only in vegetative stage leaves and flowering stage leaves.
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SOUTHERN JOURNAL OF SCIENCES

The SOUTHERN JOURNAL OF SCIENCES publishes articles in Chemistry, Physics, Mathematics, Biology, Pharmacy, Medicine, Engineering, Industrial Science, Agriculture, and related interdisciplinary areas and is intended to fill a gap in terms of scientific information worldwide. All manuscripts can be published either in English or Portuguese, with tile, abstracts, and keywords in English. At present, there are NO PUBLICATION FEES. Editors will cover web hosting, open access, DOI number, and other service costs.

We have set high standards for the articles to be published by ensuring strong but fair refereeing by at least two reviewers. We hope that this Journal will provide a forum for disseminating high-quality research in chemistry and related areas and are open to any questions and suggestions. Starting in 2020, the SOUTHERN JOURNAL OF SCIENCES will have two issues per year (June and December).

Thank you very much for choosing the SOUTHERN JOURNAL OF SCIENCES to publish your paper!
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