STATISTICAL VALIDATION OF TRIPLE COLOCALIZATION ANALYSIS
Background: in the last decades, colocalization analysis of fluorescently tagged biomolecules has proven to be a powerful approach to studying functional relationships between these biomolecules. However, in many cases, to give this analysis a biological meaning, colocalization coefficients must be tested statistically, comparing them with the colocalization expected by chance. Aim: It addressed the statistical significance of triple colocalization to distinguish real triple colocalization and classify different triple signal scenarios. Methods: we use biological and generated images of triple signal scenarios to contrast seven independent statistical facts with independent statistical tests. Three of these tests correspond to pairwise relationships (double scrambling tests), and the others correspond to triple relationships: single scrambling tests (red, green, and blue scrambling) and the triple scrambling test. The analysis and methodology proposed can be reproduced using the application developed in our laboratory. Results: In the study approach, we found true triple relationships ignored by using traditional methods of computing the statistical significance, while we could reinterpret cases of not significant triple colocalization wrongly considered as significant by traditional methods. Discussion: single scrambling tests can reveal significant triple colocalization for low levels of triple co-occurrence, even when all pairwise relationships were exclusion relationships. Moreover, on the other hand, single scrambling tests can reveal the absence of a significant triple colocalization for high levels of triple co-occurrence, even when all pairwise relationships were significant colocalization. Conclusion: all scrambling tests are useful to classify a specific scenario of a triple relationship. Dynamics like mitosis can be distinguished into their phases by triple signal relationships using these 7 independent statistical tests.
Read ArticlePRODUCTION OF BIOPLASTIC FROM POTATO STARCH
Due to their diverse properties, plastic materials are used in numerous sectors. It is possible to produce different articles and plastic objects with reduced costs, being more accessible to the population. Conventional plastics are obtained from petroleum-derived raw materials, a non-renewable resource in which their extraction and refining process cause major environmental impacts. The production of plastic reaches a level of approximately one hundred and forty million tons per year, and the disposal of these materials is increasing, generating a high rate of waste and leading to an increase of pollution since the decomposition of these materials lasts about five hundred years old. Conventional plastics can be replaced by bioplastics, a material obtained from renewable raw materials such as potatoes, cassava, maize, and which, when disposed of under favorable conditions, decomposes faster, as during its degradation process at least one step occurs. Through the metabolism of organisms present in the environment. Starch has been widely used in the production of biodegradable packaging, so the objective of this work was to produce a biodegradable bioplastic from the potato starch. Potato starch, glycerin, hydrogen peroxide, distilled water, and commercial agar were used to produce the bioplastic. Bench-scale bioplastics had good organoleptic characteristics, similar in appearance to a conventional plastic obtained from petroleum. The thickness, moisture content, and solubility of the bioplastics were analyzed, as well as their fruit preservation capacity. The samples produced were rigid and with good resistance.
Read ArticleTHE ROLE OF EDUCATION IN FOSTERING ENTREPRENEURSHIP AND INNOVATION: INSIGHTS FROM DR. BHAVNA AMBUDKAR
Background: Dr. Bhavna, an experienced leader in education and innovation, has held various leadership positions, including head of department, dean of alumni relations, and president of the institutions innovation council. She has been instrumental in initiating the alumni cell at her previous organization and establishing world-class state-of-the-art laboratories in collaboration with the automotive sector. Aims: Dr. Bhavna's efforts aim to create an ecosystem for innovation and entrepreneurship within the institute and beyond, encouraging learners to consider entrepreneurship as a career option and fostering innovation in the electronics manufacturing domain. Methods: Dr. Bhavna's approach involves raising awareness about innovation and entrepreneurship among learners, fostering collaborations between academia and industry, and establishing world-class state-of-the-art laboratories to bridge the gap between academia and industry. Results: Dr. Bhavna's efforts have resulted in a strong alumni network, with graduates across the globe contributing to various domains and positions. She has also established world-class laboratories in collaboration with the automotive sector, fostering innovation and startups in the electronics manufacturing domain. Discussions: The Ministry of Electronics and Information Technology plays a significant role in initiating innovations and startups in the electronics manufacturing domain. They have generated a separate vertical for startups, which poses challenges to innovators. If a solution is validated, the ministry handholds the innovators, incubates their ideas, funds them, and helps them reach the market at the national and international levels. Conclusions: Dr. Bhavna's vision and efforts toward fostering an ecosystem for innovation and entrepreneurship within the institute and beyond are commendable. Her experience and achievements in various leadership roles, including as the head of the department and president of the institutions innovation council, have added significant value to her professional and personal life.
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