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CARDIOVASCULAR RISK AND DIETARY HABITS IN TRANSGENDER ADULTS RECEIVING HORMONE THERAPY

Background: Transgender and gender-diverse individuals experience health inequities that may increase cardiometabolic risk. Gender-affirming hormone therapy has been associated with changes in body composition, lipid metabolism, and cardiovascular risk markers. However, limited evidence is available regarding dietary habits and cardiovascular risk profiles in transgender populations in Latin America. Objective: To evaluate cardiovascular risk factors and cardioprotective food consumption among transgender adults receiving gender-affirming hormone therapy. Methods: A quantitative, observational, descriptive, and cross-sectional study was conducted in transgender adults undergoing gender-affirming hormone therapy in Mendoza, Argentina. Eighty-seven participants completed surveys regarding sociodemographic characteristics, lifestyle habits, nutritional counseling, and consumption of cardioprotective foods. We assessed anthropometric indicators and biochemical parameters related to cardiovascular risk. Statistical analyses were performed using GraphPad Prism 8. Data were analyzed using Spearman correlation, the chi-square test, and the Mann-Whitney U test. Results: A high prevalence of modifiable cardiovascular risk factors was identified, including overweight, sedentary behavior, smoking, and low intake of cardioprotective foods. More than half of the participants had never received nutritional counseling. No statistically significant differences were observed between transgender women and transgender men in the evaluated anthropometric and biochemical variables. Nevertheless, significant correlations were identified between body mass index, waist circumference, waist-to-height ratio, and atherogenic lipid markers. Dietary quality showed limited associations with cardiovascular risk indicators, although higher dietary quality was positively associated with HDL cholesterol levels in transgender men. Conclusion: The study highlights the presence of multiple cardiovascular risk factors in transgender individuals receiving gender-affirming hormone therapy and emphasizes the importance of integrating nutritional assessment and cardiometabolic monitoring into comprehensive transgender healthcare. These findings support the need for inclusive nutritional interventions and longitudinal studies addressing cardiovascular health in transgender populations.
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DIET, SUPPLEMENTATION, PERFORMANCE, AND SUBJECTIVE EXPERIENCE IN MARATHON RUNNERS

Background: Nutrition strategies may influence marathon performance and runners’ subjective experience. Aims: To evaluate the association between dietary habits, supplement use, performance, and subjective experience in runners of the 2025 Mendoza International Marathon. Methods: A survey of 240 runners from the 2025 Mendoza International Marathon examined dietary habits, supplement use, performance, and subjective experience. Results: Nutritional counseling was linked to more favorable perceptions. Conversely, pre-race alcohol and excessive carbohydrates with poor hydration increased gastrointestinal complaints. Supplements showed no measurable performance or well-being benefits. Discussions: These findings suggest that pre-race nutritional behaviors may have a greater influence on runners’ subjective experience than supplement use.Conclusions: Nutritional guidance may improve race experience, whereas poor pre-race practices increase gastrointestinal complaints.
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CHARACTERIZATION OF TICKS IN THE GRAN MENDOZA AREA, MENDOZA, ARGENTINA

Background: Vector-borne diseases are increasingly important because they are emerging and re-emerging. Of the 867 tick species reported worldwide, 10% (86 species) have public health implications; 58 parasitize dogs, and 12 have been documented in Argentina. Tick-borne agents are increasingly relevant in the Cuyo region, with implications for both public health and domestic animal health. In Mendoza, the Rhipicephalus sanguineus complex and Amblyomma tigrinum have been documented. Aim: This study aimed to determine whether one tick genus predominated over the other, compared with previous studies in the region. Methods: We collected ticks from dogs attending municipal vaccination campaigns and veterinary clinics in several departments of the Greater Mendoza area (Ciudad/Capital, Luján de Cuyo, Godoy Cruz, and Guaymallén). Specimens were preserved in 70% (v/v) ethanol in sealed containers until analysis. Identification was performed using a stereomicroscope and the taxonomic keys of Nava. Results: The highest number of ticks was obtained from Ciudad/Capital, followed by Luján de Cuyo, Godoy Cruz, and Guaymallén. A total of 439 specimens were collected and classified by genus, species, and sex. Of these, 94.46% (415 ticks) corresponded to the Rhipicephalus sanguineus complex, including 193 females (43.96%) and 222 males (50.5%). The remaining 5.48% (24 ticks) were identified as Amblyomma tigrinum, including 17 females (3.89%) and 7 males (1.59%). Discussion: These findings confirm the marked predominance of the Rhipicephalus sanguineus complex among ticks collected from dogs in the Greater Mendoza area.
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General information

SOUTHERN JOURNAL OF SCIENCES

    General information about this journal
  • Title: SOUTHERN JOURNAL OF SCIENCES
  • Short Title: South. J. Sci.
  • ISSN: 2764-5959 (Online); ISSN: 2764-5967 (Print)
  • Universal Decimal Classification (UDC): 001
  • Review Process: Double-Blind Peer-Review
  • Accessibility: Platinum Open Access, NO-APCs.
  • Digital preservation: Portico
  • Frequency of Publication: biannual [2 issues per year]. Journal publication schedule
  • DOI: 10.48141/2764-5959
  • Website: https://www.sjofsciences.com/
  • Country: BRAZIL
  • Publisher: Mendoza University - Argentina | Araucária - Scientific Association (leaving).
  • Language of Publication: ENGLISH / PORTUGUESE* / SPANISH**
  • *Year the Journal started accepting manuscripts in Portuguese: 2020
  • *Year the Journal started accepting manuscripts in Spanish: 2026
  • First issue year: 1993
  • Free full text: Yes
  • Indexed in: Index Copernicus; Latindex, and I2OR.
  • Formerly known as the Southern Brazilian Journal of Chemistry (1993 to 2021).
  • Former ISSN: 2674-6891 (Online); Former ISSN: 0104-5431 (Print).
  • Website last update: 18/09/2026.

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ANALYTICAL METHODS FOR METHANOL DETECTION IN ALCOHOLIC BEVERAGES: A COMPARATIVE REVIEW OF CLASSICAL, COLORIMETRIC, AND CHROMATOGRAPHIC APPROACHES

Introduction: The detection of methanol in alcoholic beverages represents a critical public health issue, particularly in light of the recent outbreak of poisonings in Brazil, which registered 58 confirmed cases and 15 deaths through October 2025. Methanol's toxicity, with an estimated lethal dose ranging from 0.5 to 1.5 g/kg, requires reliable analytical methods for health surveillance. Brazilian legislation establishes a maximum limit of 20 mg/100 mL of anhydrous alcohol; however, the need for accessible screening methods in field settings remains an important challenge. Objective: To critically compare three analytical methods for methanol determination: classical qualitative methods (Lucas Test and dichromate/Schiff), Brazilian colorimetric method, and gas chromatography with flame ionization detector (GC-FID), evaluating their performance and applicability in resource-limited contexts. Methods: Theoretical-comparative approach through critical analysis of specialized literature and normative technical documentation. Methods were evaluated according to: operational principle, sensitivity (LOD/LOQ), selectivity, operational complexity, analysis time, and practical applicability. Results: The Lucas Test is not applicable for methanol detection. Colorimetric methods showed moderate sensitivity (LOD ~20-160 mg/100 mL), a 10-30-minute execution time, low operational complexity, and excellent portability. The Brazilian method presented chemical equivalence with international standards, differing only in the type of reading performed. GC-FID has shown superior sensitivity (LOD ≤ 1 mg/100 mL) and high specificity, but it requires extended time (~45-60 minutes), complex laboratory infrastructure, and specialized operators. Sugars interfere with colorimetric methods. Conclusions: The methods are complementary within a hierarchical system. Colorimetric methods enable rapid field screening, while GC-FID serves as the confirmatory method for forensic analyses. We recommend implementing integrated protocols that combine in situ colorimetric screening with GC-FID confirmation in accredited laboratories for effective health surveillance.
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SELF-ASSEMBLED MULTILAYERS OF WATER GLUCOSE MODIFIED-CHITOSAN AND GLUCOSE OXIDASE FOR DETECTION OF GLUCOSE IN MILK SAMPLES

Background: A crucial aspect of electrochemical enzymatic biosensor development is the immobilization of the enzymes, as it directly influences the sensitivity of the bioelectrode. Among the different methods used to incorporate enzymes on the surface of the transducers, layer-by-layer (LbL) self-assembly based on electrostatic interaction with polyelectrolytes of opposite charge stands out due to its simplicity and reproducibility. Aims: The aim of the work was to develop an electrochemical glucose biosensor by LbL assembly of a new functionalized chitosan polycation and the enzyme glucose oxidase (GOx). Methods: Chitosan was chemically functionalized with glucose by the Maillard reaction. The resulting polycation, named G-Chit, is soluble in the medium compatible with the enzyme. The bioelectrode was obtained by alternating adsorption of G-Chit and GOx onto carbon paste electrodes. By selecting the number of bilayer of G-Chit/GOX, the enzyme concentration, and the pH, the electroanalytical performance of the biosensor was optimized. The electrochemical responses were characterized by cyclic voltammetry and chronoamperometry. Results: Under optimized experimental conditions, the biosensor exhibited a sensitivity of (0.81 ± 0.03) µA mM-1 in a glucose concentration range of (0.18 to 1.75) mM. Discussion: Results indicated that catalytic response increases both with the number of G-Chit/GOx bilayers and the enzyme concentration, obtaining the best responses for 3 bilayers and 2 mg mL-1, respectively, while the optimum working pH value was 7.0. Conclusions: The analytical response of the biosensor was tested in milk samples with negligible matrix effects, suggesting a potential application in other dairy products. Results show that G-Chit appears promising for the immobilization of enzymes.
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TYPE 2 DIABETES MELLITUS EFFECTS ON SEMEN PARAMETERS AND SEMINAL PLASMA

Background: Diabetes Type 2 is a complex disorder described by an imbalance between insulin resistance and secretion that induce liver glucose output. It has been shown that serum insulin levels are affected by a sperm plasma membrane and acrosome. Therefore, during insulin resistance spermatogenesis changes, diabetic patients detect testicular changes. Aims: This research aims to know the effect of diabetic type 2 on some aspects of fertility in men by studying the characteristics of the semen and some biochemical parameters in seminal plasma. Methods: This study was achieved at the Center for Endocrinology and Diabetes Specialists in Maysan province from February to November 2018 and included 45 men (30 diabetic and 15 healthy in the control group) aged 30 to 59 years. The patients were divided according to age into two groups, the first (30-39) and second (40-59) years, also divided by the duration of diabetes into two groups, the first (1-5) and second (6-10) year. Results: The pH of semen in the second age group (40-49 years) group and first duration (1-5 years) group were significantly decreased (P <0.05) in diabetes compared with the control group. Volume and viscosity did not have significant differences in patients compared to the control following the age and duration of diabetes. Liquefaction only in the first age (30-39 years) group significantly increased (P <0.05) compared to the control. The concentration of sperm, progressive motility, non-progressive and normal morphology decreased (P <0.05) significantly. While the sluggish, dead, and abnormal morphology significantly increased (P <0.05) in all diabetes groups compared with the control. The fructose and alkaline phosphatase values in the seminal plasma were not differing significantly in patients compared with the control. Zinc and glutathione values decreased significantly (P <0.05) compared with control in each age and duration of diabetes. Discussion: Insulin stimulates the Ledying cell function, defect insulin effect on spermatogenesis. Impaired sperm motility in a patient with D.M. might be attributed to many reasons, such as increased ROS level, altered mitochondria DNA, and decreased epididymal products. Conclusion: our measurement indicates that there is an effect of type 2 diabetes mellitus on semen parameters and seminal plasma biochemical parameters.
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SOUTHERN JOURNAL OF SCIENCES

The SOUTHERN JOURNAL OF SCIENCES publishes articles in Chemistry, Physics, Mathematics, Biology, Pharmacy, Medicine, Engineering, Industrial Science, Agriculture, and related interdisciplinary areas and is intended to fill a gap in terms of scientific information worldwide. All manuscripts can be published either in English or Portuguese, with tile, abstracts, and keywords in English. At present, there are NO PUBLICATION FEES. Editors will cover web hosting, open access, DOI number, and other service costs.

We have set high standards for the articles to be published by ensuring strong but fair refereeing by at least two reviewers. We hope that this Journal will provide a forum for disseminating high-quality research in chemistry and related areas and are open to any questions and suggestions. Starting in 2020, the SOUTHERN JOURNAL OF SCIENCES will have two issues per year (June and December).

Thank you very much for choosing the SOUTHERN JOURNAL OF SCIENCES to publish your paper!
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