HIGH BURDEN OF VITAMIN D DEFICIENCY AND FERRITIN-LINKED IMPACT IN Β-THALASSEMIA MAJOR
Background: Background: Vitamin D plays an essential role in bone health and overall physiological function, and its deficiency is common in children and adolescents with β-thalassemia major (βTM). Iron overload, as reflected by elevated ferritin, may further influence vitamin D status. Aim: This study aimed to evaluate serum vitamin D levels in βTM patients and determine their association with ferritin levels. Methods: A total of 40 βTM patients and 20 age-matched healthy controls (aged 4–25 years) were enrolled between October 2024 and February 2025. Serum vitamin D, calcium, ferritin, and hemoglobin were measured. Statistical analysis, including correlation and logistic regression, was performed using SPSS v.26 to identify predictors of vitamin D deficiency. Results: Vitamin D deficiency was highly prevalent among βTM patients (70%) compared with controls. Patients showed significantly lower vitamin D levels (17.32±1.56) than controls (25.34±1.76). Vitamin D levels were positively correlated with age (r = 0.788), calcium (r = 0.772), and hemoglobin (r = 0.771), and negatively correlated with ferritin (r = −0.517). Logistic regression demonstrated that ferritin >1000 ng/mL strongly predicted vitamin D deficiency (OR = 17.875; 95% CI: 3.258–98.074; p = 0.001), while younger age ( < 10 years) also increased the odds of deficiency (OR = 5.200; p = 0.018). Discussion: D deficiency is a prevalent and intrinsic metabolic disturbance in β-thalassemia major, closely linked to chronic iron overload and elevated ferritin levels. This interplay disrupts hepatic vitamin D hydroxylation, induces inflammation, and contributes to endocrine and skeletal complications, highlighting ferritin as a key predictor of deficiency in these patients. Conclusion: Vitamin D deficiency is highly prevalent in βTM and is strongly associated with elevated ferritin levels, suggesting that iron overload is a significant predictor. Integrating vitamin D assessment into routine monitoring may support better management of disease-related metabolic disturbances in patients with βTM.
Read ArticleADVANCES IN NATURAL EXTRACTS USED FOR ANTIBIOTIC-RESISTANT BACTERIA TREATMENT: THE GRAM-NEGATIVE CASES
Background: Infectious diseases are a global problem, the second human cause of death. Infectious diseases caused by pathogenic bacteria have been treated with a high degree of efficacy. However, even when the 20th century was considered the “golden age” of antibiotics, bacteria developed a different resistance mechanism to antibiotics. In 2017, the WHO issued an alert about 12 bacteria with an urgent need to develop new antibiotics. Aims: The aim of the present review is to analyze the current knowledge of the antibacterial activity of natural extract-based treatments against the pathogens listed by WHO. Methods: A systematic review of the literature in PubMed was performed to search for publications describing the use of natural extracts as antibiotics over bacteria. We focused on the Gram-Negative group. The exclusion criteria consisted of limiting papers on natural extracts tested over the bacteria culture related to eight selected bacteria, according to an alert issued by WHO in 2017, and seven plant extracts. Results: All the Gram-Negative bacteria listed in 2017 by WHO have been treated, with different degrees of advance, with some of the plant extracts and plant-based compounds reviewed. In general, the first approach is using inhibition disks applied over the bacterial biofilm in solid culture media. Discussion: While Salmonellae and P. aeruginosa have been extensively studied, over N. gonorrhoeae, A. baumannii have been tested with fewer natural extracts. Edible herbs are more often used, as well as artemisa and wine byproducts. In all cases, they are in the early stages of study, not being tested in patients at present. Conclusions: Plant extracts and plant-based compounds are effective as antibacterial, with minimal effects on the host cell. Furthermore, they are sustainable, environmentally friendly, and renewable.
Read ArticleDENSE CLUSTERS OF RAW SEWAGE LOCATIONS ON MINNA NEIGHBOURHOOD GIS MAPS ARE POINTERS TO VERITABLE URBAN DECAY AND MOSQUITO-BREEDING GROUNDS
Background: The lack of comprehensive baseline data on raw sewage pollution in Minna, Nigeria's Niger State capital, represents a significant gap in public health information. This deficiency necessitates a systematic study to establish a database documenting sewage pollution patterns in Minna's urban environment. Aim: To employ georeferencing tools and descriptive observations for identifying and mapping potential mosquito breeding sites related to sewage discharge within Minna's built-up areas and to develop an interactive Geographic Information System (GIS) map as an environmental audit tool for public health officials. Methods: The study area was divided into five sectors: Greater Bosso, Minna Central, Greater Maitumbi, Tunga, and Greater Chanchaga. Following initial site familiarization and GIS equipment testing, systematic surveys were conducted. Field teams documented locations of household sewage discharge points, collecting geographic coordinates, temporal data (date, time, weather conditions), and site characteristics. Each identified location was photographed and recorded in standardized data sheets, including household information where available. Results: Using ArcGIS®10.8 software, comprehensive sewage pollution layers were created for each sector by integrating collected field data with Minna's township built-up and settlement shapefiles. The mapping revealed distinct pollution patterns and clusters across different neighborhoods. Discussion: Analysis of the spatial distribution showed a clear correlation between socioeconomic status and sewage management practices, with higher concentrations of improper sewage discharge in low-income areas. Conclusions: This database serves as a valuable resource for public health interventions targeting mosquito breeding grounds. Regular monitoring through periodic sewage pollution audits and expanded geographical coverage is recommended for improved urban health management.
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