SOUTHERN JOURNAL OF SCIENCES

Of the most fundamental fields of modern biology is transcriptomics, with a focal point on the expression pattern of plants under various conditions by assessing ribonucleic acid. So far, this approach has been a game-changer in revealing the gene structure, function, and most importantly, their cellular and biological role. Considering the criticality of pathogens for crop plants, understanding plant defense mechanisms against them is in high demand. This study aimed to review the principles of these approaches and their recent application in the plant. An Important method to address this gap is transcriptomics, which can effectively provide insight into plants against pathogens. This field has covered different aspects of plant biology besides the plant-pathogen relationship. Identifying pathogens in infected plants and the series of reactions they provoke at the gene level is crucial to finding the responsible gene (s). Finding the gene associated with resistance or vulnerability to a specific pathogen paves the way to differentiate the potential genotypes. Thus, the breeding attempts would be more successful. The advancement in biotechnology has revolutionized this field with some of the methods that have been commonly applied in studies on the plant-pathogen relationship, for instance, Northern blotting, microarray, real-time polymerase chain reaction.

Pruning methods can primarily affect grape production at various levels. Of the commonly affected features by pruning, the number of bunches has known to have a significant effect on the relation between the functions of assimilate sources and sinks. Further, fruitfulness can significantly be negatively influenced by shading. In this study, we aim to study the impact of 4, 6 and 8 buds per cane with pruning intensity as light, moderate and severely pruned and their interaction in the course two consecutive years, 2017 and 2018 in ‘Sahebi’ and ‘Halagho’ (both seeded red table grape cultivars). In 2018, lightly pruned ‘Halagho’ and having 8 buds per cane increased number of bunches by 54.00, which had the highest value. Results showed that there is apical buds bear more fruit by comparison to the buds with vegetative nature, 1 to 4 first buds. Based upon the outcomes of this study, it could be concluded that due to the stresses imposed by the pruning of the vines, therefore, the chemical compounds increases in order alleviate the negative aftermaths . In 2018, whole berry antioxidant activity in both cultivars was significantly lessened, and this decline was higher in ‘Saahebi’. The number of bunches was increased by increasing cane length or bud load/cane. Overall, the pruning program has an impactful role in bunch number as well as antioxidant activity and in the long-run on accumulated reserves, which makes choosing and applying a specific pruning method undeniably important. Thus, emphasize the importance of further research in this field.

Background: Despite innovations in orthopedic dentistry, the manufacture of removable dentures belongs to the most popular orthopedic care category. Removable dentures are combined stimuli that affect the mucous membrane and neuro-receptor apparatus. Acrylic plastic prostheses, widely used in prosthetic dentistry, have a negative side mechanical, chemical-toxic, sensitizing, and thermal insulating effect on oral tissue and prosthetic impression area. This is often complicated by a violation of the biocenosis of the oral cavity, the growth of pathogenic microflora that releases toxins, especially an increase in the number of yeast colonies that irritate the oral mucosa and prosthetic stomatitis. According to the WHO, one-fifth of the world s population suffers or has suffered various candidiasis forms at least once. The worldwide increase in the incidence of the disease is primarily related to the fact that this infection is opportunistic, more than half of the world s population is a carrier of fungi of this kind, i.e., in most cases, it is an endogenous infection, which makes candidiasis different from other opportunistic mycoses. Aims: The purpose of this study was to study the prevalence of candidiasis in patients using removable dentures and to evaluate the effectiveness and prevention of candidiasis treatment. Methods: 100 patients with oral candidiasis of various age groups from 45 to 65 years were observed. Of these, 60 patients with removable plate prostheses; 40 patients with partially removable prostheses. Results and Discussion: Chronic forms of candidiasis were diagnosed in 40 patients and with exacerbation of chronic forms of candidiasis-60 people. The number of untreated carious cavities and poor hygienic condition of the oral cavity directly affects the severity of candidiasis. Acute forms of candidiasis were observed mainly in patients with high DMF and PMA indices. The severity of candidiasis depends on the degree and duration of wearing dentures and hygienic conditions - the most severe forms of invasive candidiasis were observed in the presence of removable plate prostheses, the complete absence of teeth, and the use of a prosthesis for more than 10-15 years. A combined lesion of the oral mucosa and the red border of the lips was observed mainly in patients older than 60 years. Conclusions: The presence of candidiasis in the oral cavity in patients with removable plate prostheses leads to a statistically significant change in the indicators of local immunity of the oral cavity: an increase in the concentration of serum IgG and IgA and the values of the coefficient of the balance of local immunity factors.